Philippine Health Research Registry
2016-12-20 11:22:58 Study
2016-12-20 11:44:19 Study Changes
2016-12-20 11:49:41 Study Changes
2016-12-20 11:54:32 Study Changes
2017-01-14 04:56:33 Study Changes
2017-01-14 04:59:33 Study Changes
2017-01-14 05:00:19 Study Changes
2017-01-14 05:00:32 Study Changes
2017-01-16 09:17:14 Study Changes
2017-01-16 09:17:20 Study Changes
Formulation of an Integrated System of Forensic DNA Testing for the Evaluation of Sexual Assault Cases in the Philippines
Last Updated on: 2017-01-16 09:17:20
Registry ID: PHRR170116-001462
Secondary Identification Number: None


Scientific Title

Formulation of an Integrated System of Forensic DNA Testing for the Evaluation of Sexual Assault Cases in the Philippines

Project Description

With the increasing number of reported cases of sexual assault in the Philippines, there is an urgent need for the routine incorporation of DNA evidence in criminal investigations to aid in the objective identification of offenders. To address the current absence of national guidelines for forensic DNA analysis, existing laboratory procedures were evaluated given local environmental conditions, limited infrastructure, and available resources. Recommendations were made based on the results of comprehensive and rigorously conducted validation experiments. The first part of the study demonstrated that two commercially manufactured multiplex STR systems are fit for in-house forensic casework. Secondly, Philippine population databases were established at 23 autosomal (n=167) and 23 Y-chromosomal short tandem repeat loci (n=167), which revealed that these panels of markers are highly potent for human identification. Finally, semen detection, DNA extraction, and mixture interpretation procedures conducted on actual post-coital samples were evaluated. An integrated system for DNA testing was formulated which may be used by local forensic laboratories in handling sexual assault cases. It was emphasised that only through the existence of a legislation mandating the routine collection, storage, processing, and interpretation of biological evidence can the criminal justice system fully benefit from forensic DNA testing.

NUHRA Details
NUHRA Regime NUHRA Classification NUHRA Priority
2010 - 2016 Health Technology Development Genomics/Molecular Technology
Project Duration
Start Date Duration in Months Target Completion Date Actual Completion Date
2012-06-04 25 2014-07-04 2014-07-31
Project Status

Completed

Implementing Agency (Primary Sponsor)

Name of Institution Classification Region LTO #
University of the Philippines - Diliman, Natural Science Research Institute, DNA Analysis Laboratory Government NCR

Cooperating Agency (Secondary Sponsor)

Name of Institution Classification Region LTO #
University of the Philippines - Los Baños Public Higher Education Institution - State Universities and Colleges Region IV-A

Funding Agency (Sources of Monetary or Material Support)

1. University of the Philippines - Diliman, Office of the Vice President for Academic Affairs
2. University of the Philippines - Los Baños, Office of the Vice Chancellor for Research and Extension
3. Commission on Higher Education

Contact for Public Queries

Name: Email Address: Phone Number: Postal Address:
Maria Corazon A. De Ungria mariadeungria@gmail.com 632 9252965 DNA Analysis Laboratory, Natural Sciences Research Institute, Miranda Hall, Quirino corner Velasquez Sts., University of the Philippines, Diliman, Quezon City

Contact for Scientific Queries

Name: Email Address: Phone Number: Postal Address:
Maria Corazon A. De Ungria mariadeungria@gmail.com 632 9252965 DNA Analysis Laboratory, Natural Sciences Research Institute, Miranda Hall, Quirino corner Velasquez Sts., University of the Philippines, Diliman, Quezon City

Investigating Team

Name Expertise Affiliation
Maria Corazon A. De Ungria, PhD Forensic Genetics University of the Philippines - Diliman, Natural Science Research Institute, DNA Analysis Laboratory

Health Condition(s) or Problem(s) Studied

The increasing number of sexual assault cases being reported yearly in the Philippines necessitates that forensic DNA testing be routinely incorporated in the investigations. This study was conducted to evaluate existing procedures for DNA testing of post coital samples and to put forward recommendations for local forensic DNA laboratories.

Primary Outcomes

(1)   PowerPlex® 21 and Y23 Systems, were demonstrated to be fit for routine forensic casework. Both kits were shown to be sensitive to down to 125 pg of DNA and can generate the full profile of the minor contributor in majority of 1:9 and 9:1 DNA mixtures. PPY23 is specific to male DNA and can generate the full profile of the male contributor down to 0.125:100 ratio of mixed male/female DNA.

(2)   Instrument-specific data interpretation thresholds were determined using methods that minimise average error rates. These include analytical, stochastic, and heterozygous peak height ratio thresholds.

(3)   An autosomal DNA database has been established for the Philippine population (n=167) at 23 short tandem repeat loci. Markers do not show significant departures (p≤0.05) from Hardy-Weinberg equilibrium and linkage equilibrium. The combined power of discrimination and combined match probability for the complete marker set are 0.999999999999999999999999999179 and 1 in 1.22 x 102, respectively.

(4)   A Y-chromosomal haplotype database has been established for the Philippine population (n=167) at 23 Y-STR loci. The complete marker panel was able to individuate all males in the database, resulting to 167 unique profiles. These were uploaded and made publicly available in the Y-chromosome Haplotype Reference Database (www.next.yhrd.org).

(5)   Presumptive tests kits for semen were compared, namely Seminal Fluid DISCHAPSTM, an acid phosphatase test kit, and Mini BLUEMAXX™ III, an alternate light source method using 455 nm light. Both procedures can detect semen down to 1:8 dilution. Whilst the AP test exhibited greater specificity to semen, the use of Mini BLUEMAXX™ III is less tedious and less susceptible to sample contamination.

(6)   RSIDTM-Semen is a highly sensitive confirmatory test for semen. It can detect

(7)   The standard phenol-chloroform (organic) method generally resulted to higher DNA yields compared to the silica-based QIAamp DNA Micro procedure. However the latter generated more balanced profiles, exhibited higher success of recovering DNA from the semen contributor, produced fewer wastes, and was less tedious.

(8)   Partial to full profiles were obtained from swab samples stored in the WhatmanTM FTATM Indicating Elute Cards, thus the procedure requires further optimisation.

(9)   Fully continuous models implemented in expert systems such as STRmixTM, enable likelihood ratio calculations of complicated DNA mixtures and supersede traditional binary models for mixture interpretation.

Key Secondary Outcomes

None

Date of First Enrollment

2012-06-04

Recruitment Status

Completed

Countries of Recruitment

Philippines

Research Classification

Non-clinical Studies

Project Location & Institutional Ethics Review Board Which Approved the Study

Unspecified

FDA Document Tracking Number

Unspecified

FDA / ERC Approval Date

Unspecified

Amendment Approval Dates/Reasons

None

Key Inclusion and Exclusion Criteria (CT)

None

Study Type

Unspecified

Method of Allocation

Unspecified

Masking / Blinding

Unspecified

Masking Details

None

Assignment

Unspecified

Purpose

Unspecified

Phase

Unspecified

Target Sample Size (Philippines)

Actual Sample Size (Philippines)

Date of first enrollment

2012-06-04

Have you applied for Intellectual Property Protection for your product?

No

Research Utilization

Type of Research Utilization Utilization Information
Publication
Manuscript in preparation. Jae Joseph Russell B. Rodriguez, Gayvelline C. Calacal, Rita P. Laude, and Maria Corazon A. De Ungria. Evaluation of semen detection procedures for DNA testing of post-coital samples Science and Justice
 
Manuscript submitted. Jae Joseph Russell B. Rodriguez, Jo-anne Bright, Jazelyn M. Salvador, and Maria Corazon A. De Ungria. Statistical approaches to interpreting autosomal DNA mixtures obtained from post-coital samples. Forensic Science International: Genetics
 
17 October 2016 (Manuscript accepted). Jae Joseph Russell B. Rodriguez, Gayvelline C. Calacal, Rita P. Laude, and Maria Corazon A. De Ungria. Non-differential DNA extraction of post-coital samples submitted for investigating sexual assault cases in the Philippines. International Journal of Philippine Science and Technology
 
July 2015. Jae Joseph Russell B. Rodriguez, Jazelyn M. Salvador, Gayvelline C. Calacal, Rita P. Laude, and Maria Corazon A. De Ungria. Allele frequencies of 23 Autosomal Short Tandem Repeat Loci in the Philippine Population. Legal Medicine (Tokyo). (4): 295-297
 
28 April 2014. Josephine Purps, Sabine Siegert, Sascha Willuweit, Marion Nagy, Cíntia Alves, Renato Salazar, Sheila M.T. Angustia, Lorna H. Santos, Katja Anslinger, Birgit Bayer, Qasim Ayub, Wei Wei, Yali Xue, Chris Tyler-Smith, Miriam Baeta Bafalluy, Begoña Martínez-Jarreta, Balazs Egyed, Beate Balitzki, Sibylle Tschumi, David Ballard, Denise Syndercombe Court, Xinia Barrantes, Gerhard Bäßler, Tina Wiest, Burkhard Berger, Harald Niederstätter, Walther Parson, Carey Davis, Bruce Budowle, Helen Burri, Urs Borer, Christoph Koller, Elizeu F. Carvalho, Patricia M. Domingues, Wafaa Takash Chamoun, Michael D. Coble, Carolyn R. Hill, Daniel Corach, Mariela Caputo, Maria E. D’Amato, Sean Davison, Ronny Decorte, Maarten H.D. Larmuseau, Claudio Ottoni, Olga Rickards, Di Lu, Chengtao Jiang, Tadeusz Dobosz, Anna Jonkisz, William E. Frank, Ivana Furac, Christian Gehrig, Vincent Castella, Branka Grskovic, Cordula Haas, Jana Wobst, Gavrilo Hadzic, Katja Drobnic, Katsuya Honda, Yiping Hou, Di Zhou, Yan Li, Shengping Hu, Shenglan Chen, Uta-Dorothee Immel, Rüdiger Lessig, Zlatko Jakovski, Tanja Ilievska, Anja E. Klann, Cristina Cano García, Peter de Knijff, Thirsa Kraaijenbrink, Aikaterini Kondili, Penelope Miniati, Maria Vouropoulou, Lejla Kovacevic, Damir Marjanovic, Iris Lindner, Issam Mansour, Mouayyad Al-Azem, Ansar El Andari, Miguel Marino, Sandra Furfuro, Laura Locarno, Pablo Martín, Gracia M. Luque, Antonio Alonso, Luís Souto Miranda, Helena Moreira, Natsuko Mizuno, Yasuki Iwashima, Rodrigo S. Moura Neto, Tatiana L.S. Nogueira, Rosane Silva, Marina Nastainczyk-Wulf, Jeanett Edelmann, Michael Kohl, Shengjie Nie, Xianping Wang, Baowen Cheng, Carolina Núñez, Marian Martínez de Pancorbo, Jill K. Olofsson, Niels Morling, Valerio Onofri, Adriano Tagliabracci, Horolma Pamjav, Antonia Volgyi, Gusztav Barany, Ryszard Pawlowski, Agnieszka Maciejewska, Susi Pelotti, Witold Pepinski, Monica Abreu-Glowacka, Christopher Phillips, Jorge Cárdenas, Danel Rey-Gonzalez, Antonio Salas, Francesca Brisighelli, Cristian Capelli, Ulises Toscanini, Andrea Piccinini, Marilidia Piglionica, Stefania L. Baldassarra, Rafal Ploski, Magdalena Konarzewska, Emila Jastrzebska, Carlo Robino, Antti Sajantila, Jukka U. Palo, Evelyn Guevara, Jazelyn Salvador, Maria Corazon De Ungria, Jae Joseph Russell Rodriguez, Ulrike Schmidt,  Nicola Schlauderer, Pekka Saukko, Peter M. Schneider, Miriam Sirker, Kyoung-Jin Shin, Yu Na Oh, Iulia Skitsa, Alexandra Ampati, Tobi-Gail Smith, Lina Solis de Calvit, Vlastimil Stenzl, Thomas Capal, Andreas Tillmar, Helena Nilsson, Stefania Turrina, Domenico De Leo, Andrea Verzeletti, Venusia Cortellini, Jon H. Wetton, Gareth M. Gwynne, Mark A. Jobling, Martin R. Whittle, Denilce R. Sumita, Paulina Wolańska-Nowak, Rita Y.Y. Yong, Michael Krawczak, Michael Nothnagel, Lutz Roewer. 2014. A global analysis of Y-chromosomal haplotype diversity for 23 STR loci. Forensic Science International: Genetics. 12: 12-23
Oral Presentation
2 March 2016. Jae Joseph Russell B. Rodriguez. Formulation of an Integrated System of Forensic DNA Testing for the Evaluation of Sexual Assault Cases in the Philippines. Terminal report presented during the 2016 OVPAA Symposium for CWRG and ECWRG Recipients held in the Institute of Environmental Science and Meteorology (IESM) Auditorium, National Science Complex, UP Diliman
 
11 August 2015. Jae Joseph Russell B. Rodriguez. Establishment of Autosomal and Y-Chromosomal STR DNA Databases of the Philippine Population. Lecture presented during the DNA Analysis Laboratory Project Completion Seminar held at the Natural Sciences Research Institute, Conference Room, Miranda Hall, UP Diliman 
 
16 June 2015. Jae Joseph Russell B. Rodriguez. An Integrated System of Forensic DNA Testing for Sexual Assault Evidence in the Philippines. Lecture presented during the NSRI Seminar Series held at the Natural Sciences Research Institute, Conference Room, Miranda Hall, UP Diliman 
 
9 June 2015. Jae Joseph Russell B. Rodriguez. Establishment of Autosomal and Y-Chromosomal STR DNA Databases of the Philippine Population. Lecture presented during the DNA Analysis Laboratory Project Completion Seminar held at the Natural Sciences Research Institute, Conference Room, Miranda Hall, UP Diliman
 
28 November 2014. Jae Joseph Russell B. Rodriguez, Jazelyn M. Salvador, Gayvelline C. Calacal, Rita P. Laude, and Maria Corazon A. De Ungria. “Formulating an Integrated System of Forensic DNA Testing for Sexual Assault Evidence in the Philippines”. Oral paper presented in the 14th National Genetics Symposium held at Southeast Asian Fisheries Development Center (SEAFDEC), Tigbauan, Iloilo on November 27-29, 2014, with the theme “Genomics in Agriculture and Health for a Better Philippines”.
 
17 November 2014. Jae Joseph Russell B. Rodriguez, Jazelyn M. Salvador, Gayvelline C. Calacal, Rita P. Laude, and Maria Corazon A. De Ungria. An Integrated System of Forensic DNA testing for Sexual Assault Cases in the Philippines”. Oral paper presented in the 7th UPLB-CAS Student-Faculty Research Conference, College of Arts and Sciences Auditorium,  University of the Philippines Los Baños
 
11 November 2013. Jae Joseph Russell B. Rodriguez, Jazelyn M. Salvador, Gayvelline C. Calacal, Maria Lourdes D. Honrado, Minerva S. Sagum, Rita P. Laude, and Maria Corazon A. De Ungria. Comparative Validation of Semen Detection Tests: Sensitivity, Specificity and Compatibility with DNA Profiling Procedures for Mixture Samples. Oral paper presented during the 5th Annual Meeting and Symposium of the Asian Forensic Sciences Network held at Resorts World Sentosa, Singapore