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Project ADDS: detecting amplified DNA products from thermocycling

PHRR201209-003080

ADDS: Development of an Amplified DNA Detection System (ADDS) for the Detection of SARS-Cov-2 based on the Thermocycler Output of FDA-approved COVID-19 RT-PCR kits

Project ADDS aims to develop an amplified DNA detection system for detecting sars-cov-2 based on low-cost lateral flow diagnostic (LFD) strips to selectively detect the amplified viral nucleic acids that are the output of the miPCR device and/or other thermocyclers. 

Regime Classification Priority
2017 - 2022 Global competitiveness and innovation in health Biomedical products and engineering
Start Date Duration in Months Target Completion Date Actual Completion Date
2020-06-10 12 2021-06-10 0000-00-00

Ongoing

Institution Classification Region LTO #
Ateneo de Manila University Private Higher Education Institution - Sectarian NCR
Institution Classification Region LTO #
Ateneo de Manila University Private Higher Education Institution - Sectarian NCR
Institution Region
Philippine Council for Health Research and Development, Department of Science and Technology NCR
Name E-Mail Institution and Institution Address
Jeremie De Guzman jedeguzman@ateneo.edu CTC 201 Ateneo de Manila University, Katipunan Ave., Loyola Heights, Quezon City 1108
Name E-Mail Institution and Institution Address
Jeremie De Guzman jedeguzman@ateneo.edu CTC 201 Ateneo de Manila University, Katipunan Ave., Loyola Heights, Quezon City 1108
Name Expertise Affiliation
Jeremie E. De Guzman Medical Device Design, Medical Device Regulations, Public Health, Health Informatics Ateneo de Manila University, Ateneo School of Medicine and Public Health
Keith J. Moore Biochemistry, Translational Research, Pharmaceutical development Ateneo de Manila University

The ADDS project was developed to explore possible methods and techniques to detect Sars-CoV-2 genetic material without using the costly qPCR machine as a reader. 

The primary outcome of the ADDS project is to fabricate a proof of concept SARS-COV-2 qPCR detection kit with and exploring compatibility with lateral flow devices (LFD) to detect PCR amplification products. This technology potentially allows the detection of Sars-CoV-2 genes without the need for an expensive qPCR machine. In addition, the project is also exploring other methods and techniques related to tube-based fluorescence detection and a 96-well plate ELONA strategy.

Unspecified

Completed

Non-clinical Studies

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