DEVELOPMENT OF A CELL-BASED IMMUNOASSAY FOR CORONAVIRUS DISEASE 2019 (COVID-19) SEROLOGIC TESTING
PHRR211103-004042
DEVELOPMENT OF A CELL-BASED IMMUNOASSAY FOR CORONAVIRUS DISEASE 2019 (COVID-19) SEROLOGIC TESTING
The pandemic of coronavirus disease 2019 (COVID-19) caused severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has become a major global health concern. Current confirmation of COVID-19 infection is based on testing of appropriate respiratory samples using nucleic acid amplification method of reverse transcription-quantitative polymerase chain reaction (RT-qPCR). Though this method is validated, specific and faster than virus isolation, establishment of a laboratory with appropriate biosafety clearance is warranted to conduct testing. In addition, several diagnostic inconsistencies have been reported with this method. Serological testing primarily detecting virus-specific IgM and IgG antibodies in paired serum samples (i.e. in acute and convalescent phase) has been suggested to support diagnosis of COVID-19 in cases where negative nucleic acid-based amplification tests are negative but have strong epidemiological link. Some recommend its use to aid in diagnosis of subclinical cases of COVID-19 and to determine the true extent of COVID-19 spread. In this study, we propose a low-cost, cell-based immunoassay to detect antibodies against SARS-CoV-2 (AbaCoVs) without the need for sophisticated equipment or electricity.
| Regime | Classification | Priority |
|---|---|---|
| 2017 - 2022 | Global competitiveness and innovation in health | Access to essential medical products, vaccines and technologies |
| Start Date | Duration in Months | Target Completion Date | Actual Completion Date |
|---|---|---|---|
| 2021-02-15 | 12 | 2022-02-15 | 0000-00-00 |
Ongoing
| Institution | Classification | Region | LTO # |
|---|---|---|---|
| University of the Philippines Manila | Public Higher Education Institution - State Universities and Colleges | Philippines | n/a |
| Institution | Classification | Region | LTO # |
|---|---|---|---|
| University of the Philippines Manila | Public Higher Education Institution - State Universities and Colleges | Philippines |
| Institution | Region |
|---|---|
| DOST-PCHRD | NCR |
| Name | Institution and Institution Address | |
|---|---|---|
| Riziel Hannah B. Aguimatang | rbaguimatang@up.edu.ph | University of the Philippines Manila, Malate, Manila |
| Name | Institution and Institution Address | |
|---|---|---|
| Fresthel Monica M. Climacosa | fmclimacosa@up.edu.ph | University of the Philippines Manila, Malate, Manila |
| Name | Expertise | Affiliation |
|---|---|---|
| David R. McMillen | Chemistry | University of Toronto |
| Fresthel Monica M. Climacosa, MD, PhD | medical microbiology, humoral immunity, reemerging infectious diseases | University of the Philippines - Manila, College of Public Health |
| Jose Ma. M. Angeles, RMT, MPH, PhD | molecular biology, serological diagnostics (schistosomiasis) | University of the Philippines - Manila, College of Public Health, Department of Parasitology |
| Ma. Cynthia J Goh | Chemistry | University of Toronto |
| Pilarita T. Rivera MD, PhD | Immunology, molecular biology, parasitic disease | University of the Philippines - Manila, College of Public Health |
| Ruby Anne N. King, MD, PhD | polymeric peptides, antibodies | Biomedical Innovations Research for Translational Health Science Laboratory |
| Salvador Eugenio C. Caoili, MD, PhD | anitbodies, epitope prediction, vaccines | Biomedical Innovations Research for Translational Health Science Laboratory |
Coronavirus Disease(COVID-19)
- To identify candidate SARS-CoV-2 antigens for immunoassay-based detection of AbaCoVs
- To design plasmid expression vectors containing selected SARS-CoV-2 antigens for cell-surface display on yeast cells
- To develop cell-surface engineered yeast that display SARS-CoV-2 proteins
- To use cell-surface engineered yeast that display SARS-CoV-2 proteins in an immunoassay to detect AbaCoVs
Unspecified
Completed
- Philippines
Non-clinical Studies