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Application of radioimmunoassay methods for malaria detection in two selected endemic areas in the Philippines.


Erlinda S. Natera ,
Cielo J. Pasay,
 Tiu WU

Publication Information

Publication Type
Publication Sub Type
Journal Article, Original
Philipp Tech J
Publication Date
July-September 1995


Radioimmunoassay (RIA) technique was used with the synthetic peptide, (NANP) 3 in detecting anti-sporozoite antibody (against Plasmodium falciparum) in serum of persons residing in two (2) endemic areas in the Philippines. Entomological surveys for sporozoite detection in mosquito vectors utilizing monoclonal antibodies (2A10 for P. falciparum and 2F2 for P. vivax) were likewise conducted in the same areas where serological surveys were performed. These two areas are located on separate islands, with varying malaria transmission seasons and levels of endemicity Initial findings showed positive response to thh CSP antigen (NANP)3 in detecting anti - P. falciparum antibodies in sera. Infection with sporozoites of P. falciparum and P. vivax in mosquito vectors were detected using monoclonal antibodies 2A10 and 2F2 respectively. The latter procedure was shown to be more sensitive than dissection of mosquito salivary glands Initial study shows a heightened level of anti-(NANP)3 antibodies in both populations prior to the generally accepted peak of malaria season indicating that RIA with CSP antigen and specific MAbs can be a useful epidemiological tool for understanding the dynamics of malaria transmission as well as in monitoring control programmes based on reducing manvector contact.


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Philippine Council for Health Research and Development Box No. 12 Fulltext pdf (Request Document)

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